P-61: Effects of Acrylamid on Sperm Parameters and Chromatin Quality in Mice

Background: Acrylamid (AA) is found in carbohydraterich foods that are prepared at high temperatures, such as French fries and potato chips. The toxic effects of AA in male animals include degeneration of epithelial cells of seminiferous tubules, decreased number of sperm and elevation in abnormal spermatozoa. The aim of this study was to evaluate the detrimental effects of AA on sperm parameters (count, motility, viability and morphology) and chromatin quality in mice. Materials and Methods: 16 adult male mice were divided equally into two groups each conaining 8 animals .The mice of group 1 served as controls and fed on basal diet, whereas, group 2 received basal diet and acrylamide (10 mg/kg, water solution) for 35 days. Then the tail of epididymis was incised and placed in Ham’s F10 under aseptic condirion. Released spermatozoa were used to analyze number, motility, morphology (Papstaining) and viability (eosin-Y staining). For sperm chromatin condensation and DNA integrity assessments, the smears were stained by standard cytochemical techniques including acridine orange (AO), aniline blue (AB), toluidine blue (TB). Results: In AA-treated mice, a statistically significant decrease was found in sperm concentration, sperm motility, sperm viability and sperm morphology when compared with controls. In addition, a significant increase was found in sperm AB-reacted sperm cells (immature sperm), and AO-reacted spermatozoa (single-stranded DNA) in AA-treated mice compared to control animals Conclusion: Acrylamide reduced sperm parameters as well as sperm chromatin quality and DNA integrity in mice.